Pharmacokinetics and Isolated Hepatocyte
نویسندگان
چکیده
منابع مشابه
The isolated hepatocyte preparation: 30 years on.
A method for the preparation of intact rat hepatocytes in high yield was first described in 1969. The procedure involved digestion of hepatic tissue by perfusion of the liver with crude collagenase; later, purified collagenase without other enzymic additions was shown to be ineffective. Recently it has been discovered that the combination of purified collagenase plus elastase is superior to cru...
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OBJECTIVES/HYPOTHESIS Previous animal studies demonstrated that hepatocyte growth factor (HGF) has the potential to regenerate scarred vocal folds. In addition, HGF is now produced under a good manufacturing practice (GMP) procedure. Therefore, human clinical trials of HGF are warranted in patients with vocal fold scarring. In the current study, we investigated the pharmacokinetics and the loca...
متن کاملHepatic pyruvate kinase. Quantitative measurements of phosphorylation in vitro and in the isolated rat hepatocyte.
The phosphorylation of rat liver pyruvate kinase was studied in isolated rat hepatocytes and with the crystalline enzyme. Hepatocytes were incubated with ["PI orthophosphate and isotopically labeled pyruvate kinase was quantitatively isolated using a resin-coupled antibody. The molar extent of phosphorylation of the enzyme was calculated from the amount of radioactivity in the enzyme and the sp...
متن کاملInteraction of Probenecid with Methotrexate Transport and Release in the Isolated Rat Hepatocyte in Suspension1
Probenecid has been shown to delay the plasma clearance of methotrexate in the rat and to reduce both hepatic and renal excretion of methotrexate in this animal model. In order to probe the mechanism by which probenecid alters hepatic excretion of the antifolate, studies assessed the effects of probenecid on transport, accumulation, distribution, and release of methotrex ate in the rat hepatocy...
متن کاملDuck HBV DNA copy numbers in isolated hepatocyte nuclei vary dramatically and decline during entecavir therapy.
BACKGROUND We aimed to develop a quantitative assay to measure duck HBV (DHBV) DNA in single hepatocyte nuclei from DHBV-infected animals and to observe intranuclear DHBV DNA kinetics undergoing entecavir (ETV) therapy. METHODS DHBV DNA in isolated nuclei was amplified by quantitative real-time PCR. Liver tissues from chronically-infected ducks with or without ETV treatment were assessed. Cel...
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ژورنال
عنوان ژورنال: MOJ Bioequivalence & Bioavailability
سال: 2017
ISSN: 2573-2951
DOI: 10.15406/mojbb.2017.03.00023